Agrobacterium Protocols: Volume 1 - Methods in Molecular Biology 3rd ed. 2015 edition

Jacket Description/Back: Rapid changes and significant progress have been made in the "Agrobacterium" field, such as genetically transforming plants for both basic research purposes and agricultural development. In "Agrobacterium Protocols, Third Edition, Volumes 1 "and" 2," a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer "Agrobacterium"-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. "Volume 1" detailsupdated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: "Brachypodium distachyon, Medicago truncatula," and "Setaria viridis." It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, " ""Agrobacterium Protocols, Third Edition "facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology."Table of Contents: Part I Vectors And Model Plants1Gateway(r) Compatible Plant Transformation Vectors Mark A. Smedley and Wendy A. Harwood2Brachypodium Distachyon Jennifer N. Bragg, Amy Anderton, Rita Nieu, And John P. Vogel3Medicago Truncatula Transformation Using Cotyledonary Explants Elane Wright and Zeng-Yu Wang4Medicago Truncatula Transformation Using Leaf Explants Viviane Cosson, Alexis Eschstruth, and Pascal Ratet5Setaria Viridis Joyce Van Eck And Kerry Swartwood Part IICereal Plants6Barley (Hordeum Vulgare L.) Transformation Using Immature Embryos Cornelia Marthe, Jochen Kumlehn and Goetz Hensel 7Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures Ingrid Otto, Andrea Muller, and Jochen Kumlehn 8Maize (Zea Mays L.) Bronwyn Frame, Katey Warnberg, Marcy Main, and Kan Wang9 Maize, Tropical (Zea Mays L.) Shireen K. Assem 10Finger Millet [Eleusine Coracana (L.) Gaertn] Stanislaus Antony Ceasar, and Savarimuthu Ignacimuthu 11Oat (Avena Sativa L.) Sebastian Gasparis and Anna Nadolska-Orczyk 12Rice, Indica (Oryza Sativa L.) Yukoh Hiei, Yuji Ishida and Toshihiko Komari 13Rice, Japonica (Oryza Sativa L.) Marcy Main, Bronwyn Frame And Kan Wang 14Sorghum (Sorghum Bicolor L.) Yuechun Wan And Jeanne Layton 15Wheat (Triticum Aestivum L.) Transformation Using Immature Embryos Yuji Ishida, Masako Tsunashima, Yukoh Hiei And Toshihiko Komari 16Wheat (Triticum Aestivum L.) Transformation Using Mature Embryos Eva Medvecka And Wendy A Harwood Part IIILegume Plants17Alfalfa (Medicago Sativa L.) Chunxiang Fu, Timothy Hernandez, Chuanen Zhou, and Zeng-Yu Wang18Clovers (Trifolium Spp.) Samira Rahimi-Ashtiani, Sareena Sahab, Stephen Panter, John Mason, and German Spangenberg19Clover, Red (Trifolium Pratense) Michael L. Sullivan and Kenneth H. Quesenberry20Cowpea [Vigna Unguiculata (L.) Walp] Ratikanta Behura, Sanjeev Kumar, Bedabrata Saha, Manas Kumar Panda, Mohitosh Dey, Ayan Sadhukhan, Sagarika Mishra, Shamsher Alam, Debee Prasad Sahoo, Twinkle Sugla, Sanjib Kumar Panda, and Lingaraj Sahoo22Lentil (Lens Culinaris Medik.) Ufuk Celikkol Akcay, Meral Yucel, Huseyin Avni Oktem22Soybean [Glycine Max (L.) Merr.] Diane Luth, Katey Warnburg, and Kan Wang Part IVLegume Plants23Brassica Oleracea And B. Napus Penny A. C. Hundleby Nee Sparrow, and Judith A. Irwin24Cucumber (Cucumis Sativus L.) and Kabocha Squash (Cucurbita Moschata Duch) Yoshihiko Nanasato and Yutaka Tabei25Pepper, Chili (Capsicum Annuum) Jung Min, Sun Hee Shin, En Mi Jeon, Jung Mi Park, Ji Young Hyun, and Chee Hark Harn26Pepper, Sweet (Capsicum Annuum) Iris Heidmann And Kim Boutilier27Sugar Beet (Beta Vulgaris L.) Hiroyo Kagami, Masayuki Kurata, Hiroaki Matsuhira, Kazunori Taguchi, Tetsuo Mikami, Hideto Tamagake, and Tomohiko Kubo28Tomato (Solanum Lycopersicum) Martha L. Orozco-Cardenas, Dora Garcia, and Javier Narvaez-Vasquez "Publisher Marketing: Rapid changes and significant progress have been made in the "Agrobacterium" field, such as genetically transforming plants for both basic research purposes and agricultural development. In "Agrobacterium Protocols, Third Edition, Volumes 1 "and" 2," a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer "Agrobacterium"-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. "Volume 1" details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: "Brachypodium distachyon, Medicago truncatula," and "Setaria viridis." It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, " ""Agrobacterium Protocols, Third Edition "facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.