AFP and its Antibody Complex in Ovarian Complex

This book deals with The developed protocol for the assay of AFP for the assessment of AFP in the tissue of ovarian tumor homogenates (benign germ cell, malignant germ cell and malignant serous ). Furthermore it showsIsolation of AFP from malignant germ cell of ovarian tumor tissue homogenates by gel filtration technique, then it reveals the presence of three immunoreactive forms of AFP peak I (PI) with 592 KDa molecular weight, peak II (PII) with 190 KDa molecular weight and peak III (Pill) with 77 KDa molecular weight. kinetic studies on the binding of 1251- anti AFP antibody with AFP in tissue's homogenate of benign germ cell (01), malignant germ cell (011), malignant serous (0111) and with isolated AFP of malignant germ cell ovarian tumor (Pill) revealed that the binding reactions are time and temperature dependent. The binding data fits the second order reaction kinetics for AFP of benign germ cell group, malignant germ cell group and malignant serous group, while the binding data fits the pseudo first order reaction kinetics for isolatedAFP of malignant germ cell group. Thennodynamic studies lor the binding reaction of 1251-anti AFP antibody \vith AFP in tissue's homogenate of benign germ cell group (01}, malignant germ cell group (011), malignant serous group (0111) and with isolated AFP of malignant genn cell group (Pill), revealed that the binding reactions were exothermic and spontaneously ( and the binding reactions were entropically and enthalipically driven . Spectroscopic studies revealed that each of AFP, and the complex of isolated AFP fonn with 1251- anti AFP antibody have characteristic spectrum and give an idea of the location of particular amino acid in the AFP and ( 1251-anti AFP antibody I AFP) complex molecules