Lipid Signaling Protocols - Methods in Molecular Biology 2009 edition

Marc Notes: Includes bibliographical references and index. Jacket Description/Back: Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular Biology series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls. Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology."Table of Contents: Part I. Lipid Systems and Techniques -- 1. Eicosanoids: Generation and Detection in Mammalian Cells / Valerie B. O'Donnell, Ben Maskrey, Graham W. Taylor -- 2. Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry / Trevor R. Pettitt -- 3. Measurement of Polyphosphoinositides in Cultured Mammalian Cells / Frank T. Cooke -- 4. Inositol Lipid-Dependent Functions in Saccharomyces cerevisiae: Analysis of Phosphatidylinositol Phosphates / Stephen K. Dove, Robert H. Michell -- 5. Methods for the Determination of the Mass of Nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5) P[subscript 2] / David R. Jones, Yvette Bultsma, Willem Jan Keune, Nullin Divecha -- 6. Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin / Marie Garnier-Lhomme, Erick J. Dufourc, Banafshe Larijani, Dominic Poccia -- 7. Solution and Solid-State NMR of Lipids / Axelle Grelard, Anthony Couvreux, Cecile Loudet, Erick J. Dufourc -- 8. Spatial Localization of PtdInsP[subscript 2] in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain / Xavier Mulet, Erika Rosivatz, Ka Kei Ho, Beatrice L. L. E. Gauthe, Oscar Ces, Richard H. Templer, Rudiger Woscholski -- 9. Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes / Sebastian J. Fleire, Facundo D. Batista -- 10. Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes / F.-Xabier Contreras, Ana-Victoria Villar, Alicia Alonso, Felix M. Goni -- 11. Effects of Sterols on the Development and Aging of Caenorhabditis elegans / Eun-Young Lee, Pan-Young Jeong, Sun-Young Kim, Yhong-Hee Shim, David J. Chitwood, Young-Ki Paik -- 12. Proteomic Analysis of the Sterol-Mediated Signaling Pathway in Caenorhabditis elegans / Byung-Kwon Choi, Yun-Kyung Shin, Eun-Young Lee, Pan-Young Jeong, Yhong-Hee Shim, David J. Chitwood, Young-Ki Paik -- Part II. Lipid Metabolism and Recognition -- 13. Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt / Abdelkader Essafi, Ana R. Gomes, Karen M. Pomeranz, Aleksandra K. Zwolinska, Rana Varshochi, Ursula B. McGovern, Eric W.-F. Lam -- 14. Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt / Erika Rosivatz, Rudiger Woscholski -- 15. Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by in vitro and in vivo Assays / Lisa M. Ooms, Jennifer M. Dyson, Anne M. Kong, Christina A. Mitchell -- 16. Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic [beta]-Cells and Insulin-Responsive Muscle Cells and Adipocytes / Rosanna Cazzolli, Ping Huang, Shuzhi Teng, William E. Hughes -- 17. Protein Kinase C as an Effector of Lipid-Derived Second Messengers / Marie-Helene Paclet, Jan K. Davidson-Moncada, Guillermo Lopez-Lluch, Dongmin Shao, Lodewijk V. Dekker -- 18. Detection of Myotubularin Phosphatases Activity on Phosphoinositides in vitro and ex vivo / Holger Maria Rohde, Helene Tronchere, Bernard Payrastre, Jocelyn Laporte -- 19. Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts / Mark G. Waugh, Shane Minogue, J. Justin Hsuan -- 20. Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy / Keiichiro Kami, Sundaresan Rajesh, Michael Overduin -- 21. Revealing Signaling in Single Cells by Single- and Two-Photon Fluorescence Lifetime Imaging Microscopy / Damien Alcor, Veronique Calleja, Banafshe Larijani -- 22. Phosphoinositide (PI) 3-Kinase Assays / Michael J. Fry -- 23. Measurement of Phosphatidylinositol and Phosphatidylcholine Binding and Transfer Activity of the Lipid Transport Protein PITP / Shamshad Cockcroft -- 24. In vitro Reconstitution of Activation of PLC[epsion] by Ras and Rho GTPases / Natalia Lamuno Gandarillas, Tom D. Bunney, Michelle B. Josephs, Peter Gierschik, Matilda Katan -- 25. Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities / Jonathon R. Halstead, Mireille H. Snel, Sarah Meeuws, David R. Jones, Nullin Divecha -- 26. Preparation of Membrane Rafts / Mark G. Waugh, J. Justin Hsuan -- Index. Review Citations:

Scitech Book News 03/01/2009 pg. 72 (EAN 9781588297273, Hardcover)

Contributor Bio:  Larijani, Banafshe Banafshe Larijani. Cancer Research UK Colin A. Rosser. Rye St Anthony, Oxford, UK Rudiger Woscholski. Faculty of Natural Sciences, Imperial College, London, UK